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KMID : 0545120210310081144
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Journal of Microbiology and Biotechnology
2021 Volume.31 No. 8 p.1144 ~ p.1153
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Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187
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Zhang Qing
Wang Jie
Sun Qing
Zhang Shu-Ming
Sun Xiang-Yang
Li Chan-Yuan
Zheng Miao-Xin
Xiang Wen-Liang
Tang Jie
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Abstract
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A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp. mesenteroides. The purified rEPS, designated as rEPS414, had a uniform molecular weight of 7.757 × 105 Da. Analysis of the monosaccharide composition revealed that the molecule was mainly composed of glucose. The Fourier transform-infrared spectrum showed that rEPS414 contained both α-type and β-type glycosidic bonds. 1H and 13C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid. Scanning electron microscopy demonstrated that the exopolysaccharide displayed a large number of scattered, fluffy, porous cellular network flake structures. In addition, rEPS414 exhibited strong in vitro antioxidant activity. These results showed that strain LM187 and its rEPS are promising probiotics with broad prospects in industry.
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KEYWORD
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Characterization, released exopolysaccharide, Leuconostoc mesenteroides, antioxidant
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